UPM Institutional Repository

Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis


Tasrip, Nor Asmara and Mohd Desa, Mohd Nasir and Cheah, Yoke Kqueen and Chong, Pei Pei and Shamsudin, Mariana Nor (2012) Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis. In: 4th International Conference on the Development of Biomedical Engineering, 8-12 Jan. 2012, Ho Chi Minh, Vietnam. (pp. 135-136).


40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isolates were subjected to antibacterial assay against 2 pathogenic bacteria of gram positive and gram negative (Escherichia coli ATCC 25922 & Staphylococcus aureus ATCC 25923), by perpendicular streak method using MHA and TSA as the test media. With MHA as the test media in the antibacterial assay, > 50 % of the isolates showed antibacterial activity to at least one of the test bacteria as compared by using TSA as the test media. Color series of aerial mycelium was described based on growth on ISP-2 medium; there was a significant association between grey and non-grey color series of the aerial mycelium in association with the antibacterial activity of the isolates on MHA (89% vs. 42% respectively, P = 0.013) and TSA (78% vs. 35% respectively, P = 0.025). As a conclusion, different chemical composition of the test media could have an effect on the outcome of the antibacterial assay (MHA vs. TSA). The observed relationship between aerial mass-color (grey vs. nongrey) and antibacterial activity warrant further investigation in a larger sample of isolates.

Download File

[img] PDF
ID 27407.pdf
Restricted to Repository staff only

Download (155kB)

Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
Institute of Bioscience
DOI Number: https://doi.org/10.1007/978-3-642-32183-2_35
Notes: Full text are available at Special Collection Division Office
Keywords: Streptomyces; Antibacterial activity; Aerial mycelium; Color
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 29 Mar 2014 15:16
Last Modified: 24 Apr 2018 04:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/978-3-642-32183-2_35
URI: http://psasir.upm.edu.my/id/eprint/27407
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item