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Deciphering the function of YcbK lipoprotein-like hypo-thetical protein from Klebsiella pneumoniae MGH 78578.


Citation

Boon, Aun Teh and Choi, Sy Bing and Mohd Najimuddin, Mohd Nazalan and Salleh, Abu Bakar and A. Wahab, Habibah and Mohd Yahaya, Normi (2012) Deciphering the function of YcbK lipoprotein-like hypo-thetical protein from Klebsiella pneumoniae MGH 78578. In: Annual Symposium Frontiers in Biological Catalysis, 10-12 Jan. 2012, United Kingdom. .

Abstract / Synopsis

Klebsiella pneumoniae is a Gram negative opportunistic pathogen which causes respiratory infection among immunocompromised patients. Recently more extended-spectrum beta-lactamase-producing K. pneumoniae strains are emerging worldwide making treatment for this bacterium more difficult. Approximately 20% of the genome of K. pneumoniae MGH 78578 encodes hypothetical proteins. Hypothetical proteins are proteins with unknown function where their sequences and structures are largely non-similar with other known proteins. Deciphering the functions of hypothetical proteins of this pathogen may give insight to possible new mechanisms leading to potential drug targets in the future. In this study, a particular lipoprotein-like hypothetical protein, YcbK, from K. pneumoniae MGH 78578 was cloned and characterized. Expression of YcbK in Escherichia coli JE5505 lipoprotein- deficient strain interestingly showed the presence of film-like material on the surface of the cells. In addition, the colonies on agar plates appeared to be smoother and more opaque than the negative control. Bioinformatics analysis on YcbK revealed it contains a putative conserved domain belonging to the Peptidase M15_3 superfamily. In silico homology modeling of the protein utilizing this conserved domain revealed that it preserves a Zn binding motif with two conserved His and Asp residues important for chelation of the metal ion. All the results obtained point to the possibility of YcbK adopting the function as a metallopeptidase with an important role in cell wall architecture similar with a few other peptidases in the family.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Notes: Full text are available at Special Collection Division Office
Keywords: YcbK; Klebsiella pneumoniae MGH 78578; Hypothetical protein; Homology modelling; Cloning; In vivo characterization.
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 03 Mar 2014 09:23
Last Modified: 08 Apr 2014 17:19
URI: http://psasir.upm.edu.my/id/eprint/27345
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