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Method for isolating DNA


Citation

Mohd Ali Hassan (2008) Method for isolating DNA. UNSPECIFIED.

Abstract / Synopsis

The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.


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Additional Metadata

Item Type: Patent
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Keywords: Isolating DNA; DNA; Nucleic acid
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 04 Dec 2013 02:34
Last Modified: 08 Sep 2016 02:37
URI: http://psasir.upm.edu.my/id/eprint/26962
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