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Antiviral properties of Berembang Bukit and Kandis Hutan against psedorabies virus in animal cell culture


Goh, Sheen Yee and Allaudin, Zeenathul Nazariah and Tan, Seok Shin and Loh, Sandy Hwei San and Ting, Kang Nee and Mohd Lila, Mohd Azmi (2011) Antiviral properties of Berembang Bukit and Kandis Hutan against psedorabies virus in animal cell culture. In: 6th Seminar on Veterinary Sciences, 11-14 Jan. 2011, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 17-21).


Tropical rainforest in Malaysia represents and untapped potential source of antiviral compounds. Bioactive compounds in plant species from the same genus as Kandis Hutan such as xanthones, benzophenones, biflavonoids and lupeol had been studied. Eugeniin is an anti-herpesvirus compound which had also been found n Berembang Bukit. This preliminary study was carried out to discover the presence of antiviral properties in Berembang Bukit and Kandis Hutan using different antiviral assays. In this study, MTT cell viability assay was used in addition to microscopic evaluation of pseudorabies virus (PrV)- induced cytopathic effects (CPE) on Vero cells. The cellular toxicity of DMSO was also evaluated. DMSO was less than 10% cytotoxic at concentration of 0.1% to Vero cells and its effect can be negligible. Both plants had demonstrated antiviral properties in thyl acetate and ethanol extracts. From our findings from all three antiviral assays, the ethanol-extracted Kandis Hutan possessed the most promising antiviral properties. Nevertheless, antiviral potential of ethyl acetate and ethanol-extracted Berembang Bukit and ethyl acetate-extracted Kandis Hutan also merit further investigation

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Psedorabies virus; Plant extracts; Antiviral assays; MTT assay; DMSO; Cytotoxicity
Depositing User: Azizan Arshad
Date Deposited: 19 Nov 2013 00:35
Last Modified: 30 Oct 2019 04:12
URI: http://psasir.upm.edu.my/id/eprint/26584
Statistic Details: View Download Statistic

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