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Immuniregulatory response following fluranthene instillation in embryonated chicken eggs


Abdul Wahid, Firhanis and Mohamed Mustapha, Noordin and Mazlan, Mazlina and Md Isa, Nur Mahiza (2011) Immuniregulatory response following fluranthene instillation in embryonated chicken eggs. In: 6th Seminar on Veterinary Sciences, 11-14 Jan. 2011, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 12-16).


The immunotoxic effect the air pollutant, floranthene in chicken has never been documented. This study was undertaken to determine the possible immunotoxicity of fluoranthene in embryonated chicken eggs. Fifty 9-day-ole embryonated chicken eggs were used in this study where 40 were each inoculated with fluoranthene at the dose of 15 mg/kg via the allantoic route. The remaining 10 eggs were inoculated with phosphate buffered saline (PBS) and acted as controls. All eggs were incubated at 37°C and candled every day for evidence of embryonic survival. Dead embryos (before 21 days old) were necropsied while the allantoic fluid and yolk collected for the determination of ND-HI titers. Chicks that hatched were sacrificed and blood was collected for ND-HI titer determination and lymphoid organs were procured for histopathology. Postmoetem findins fo fluoranthene inoculated embryos were stunted growth, generalized or localized harmorrahages especially at legs and head. Histopathologically, fluoranthene induced lymphoid hyperplasia in the thymus, spleen and bursa. Such change has led to an increase in antibody production compated to the controls. This study has provided evidence that fluoranthene may cross the egg barrier (in ovo) in avian species.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Fluranthene; Embryonated eggs; Imunoregulation; ND-HI titer
Depositing User: Azizan Arshad
Date Deposited: 19 Nov 2013 00:32
Last Modified: 21 Nov 2016 07:48
URI: http://psasir.upm.edu.my/id/eprint/26582
Statistic Details: View Download Statistic

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