UPM Institutional Repository

Virullent Newcastle disease virus differentially modulates chicken splenic lymphocyte/macrophage populations and cytokine expression.


Rasoli, Mehdi and Sheau, Wei Tan and Swee, Keong Yeap and Ideris, Aini and Bejo, Mohd Hair and Kaiser, Pete and Omar, Abdul Rahman Virullent Newcastle disease virus differentially modulates chicken splenic lymphocyte/macrophage populations and cytokine expression. In: Avian Immunology Research Group Meeting (AIRG) 2012, 28-31 Aug 2012, Edinburgh, United Kingdom. .


Newcastle Disease virus (NOV) is a highly infectious virus which contributed to the major causes of economic losses in poultry industry. Since 1960 the majority of virulent NOV circulating throughout the world are of genotypes V, VI, VII, VIII and X. Since 2000 until present, genotype VII is the predominant NOV strains circulating in various parts of the world. In addition, the majority of the outbreaks are reported in NOV vaccinated flocks. Studies have been carried out to determine the possible factors contributed to the ability of the virus to break vaccine-induced immunity. However, the interaction of virulent NOV with different lymphocytes is still unclear. Understanding the virus immunoregulation on the host immune system will provide valuable information to define the molecular immunopathology of the virus. In this study, we characterize the immunoregulation of virulent NOV genotype VII and VIII on chicken macrophages, Band T lymphocytes during the acute stage of the respective virus infection in specific-pathogen-free (SPF) chickens. Spleen T! B lymphocytes and macrophage population were quantified using flow cytometry while cytokines expression was quantified using both GenomeLab GeXP system and real-time PCR assay. Results have shown that NOV from genotype VII (lBS/002) and VIII (AF2240) induced drastic reduction of C04 and COS T lymphocyte associated with infiltration of macrophage in spleen day 3 post-infection. The depletion of the T lymphocytes is primarily through the process of apoptosis. Significant upregulation of pro-inflammatory and Th-1like cytokines such as IFN-y, IL-ll3, IL-6, IL-12a and IL-B expression was also observed in the spleen of NOV infected chicken after day 3 post-infection. However, no significant changes were detected on the expression of majority of Toll-like receptor(TLR) genes. The immunoregulation effects of both virulent NOV strains on chicken splenic lymphocytes and macrophages are further studied and discussed.

Download File

[img] PDF
ID 25740.pdf
Restricted to Repository staff only

Download (183kB)

Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Subject: Newcastle disease virus - Case studies
Subject: Poultry - Diseases
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Keywords: Newcastle disease; Newcastle disease virus; Infectious virus; Poultry industry; Chicken.
Depositing User: Samsida Samsudin
Date Deposited: 21 Oct 2013 08:24
Last Modified: 27 Aug 2014 04:59
URI: http://psasir.upm.edu.my/id/eprint/25740
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item