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Molecular characterization of Pasteurella multocida isolates from rabbits


Al-Haddawi, Muthafar Hussain and Jasni, Sabri and Radu, Son and Mutalib, Abdul Rahim and Bahaman, Abdul Rani and Saad, Mohd Zamri and Sheikh Omar, Abdul Rahman (1999) Molecular characterization of Pasteurella multocida isolates from rabbits. Journal of General and Applied Microbiology, 45 (6). pp. 269-275. ISSN 0022-1260; ESSN: 1349-8037


Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and somatic type, and the anatomic site of isolation. Six isolates were found harboring plasmids. A similar ladder pattern ranging from 18 to 3 megadalton (Mda) were found in three isolates recovered from diseased rabbits. One band of molecular weight 6.6 Mda was shared by four of five (4/5) isolates from the diseased rabbits. No correlation was found between the presence of the common plasmids and serotype, resistance to antimicrobial agents, and anatomic sites from which the bacteria were cultured. Random amplification polymorphic DNA was applied to subtype all the isolates of P. multocida. Two single primers were tested for their abilities to generate individual fingerprints by using PCR. Primer 1 grouped the isolates into 7 profiles, and primer 2 grouped them into 15. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) results show the presence of a wide heterogeneity within P. multocida isolates. Therefore RAPD-PCR is an efficient technique to detect the DNA polymorphism and could be used to discriminate P. multocida of rabbit isolates together with serologic typing.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.2323/jgam.45.269
Publisher: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
Keywords: Pasteurella multocida; Plasmid; Rabbit; RAPD-PCR; Serotyping
Depositing User: Azizan Arshad
Date Deposited: 10 Oct 2013 03:56
Last Modified: 25 Apr 2016 04:44
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2323/jgam.45.269
URI: http://psasir.upm.edu.my/id/eprint/25446
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