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Attenuated Salmonella typhimurium SV4089 as a potential carrier of oral DNA vaccine in chickens.


Citation

Jazayeri, Seyed Davoud and Ideris, Aini and Zakaria, Zunita and Omar, Abdul Rahman (2012) Attenuated Salmonella typhimurium SV4089 as a potential carrier of oral DNA vaccine in chickens. Journal of Biomedicine and Biotechnology, 2012 (264986). pp. 1-8. ISSN 1110-7243

Abstract

Attenuated Salmonella has been used as a carrier for DNA vaccine. However, in vitro and in vivo studies on the bacteria following transfection of plasmid DNA were poorly studied. In this paper, eukaryotic expression plasmids encoding avian influenza virus (AIV) subtype H5N1 genes, pcDNA3.1/HA, NA, and NP, were transfected into an attenuated Salmonella enteric typhimurium SV4089. In vitro stability of the transfected plasmids into Salmonella were over 90% after 100 generations. The attenuated Salmonella were able to invade MCF-7 (1.2%) and MCF-10A (0.5%) human breast cancer cells. Newly hatched specific-pathogen-free (SPF) chicks were inoculated once by oral gavage with 109 colony-forming unit (CFU) of the attenuated Salmonella. No abnormal clinical signs or deaths were recorded after inoculation. Viable bacteria were detected 3 days after inoculation by plating from spleen, liver, and cecum. Fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) were carried out for confirmation. Salmonella was not detected in blood cultures although serum antibody immune responses to Salmonella O antiserum group D1 factor 1, 9, and 12 antigens were observed in all the inoculated chickens after 7 days up to 35 days. Our results showed that live attenuated S. typhimurium SV4089 harboring pcDNA3.1/HA, NA, and NP may provide a unique alternative as a carrier for DNA oral vaccine in chickens.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1155/2012/264986
Publisher: Hindawi Publishing Corporation
Keywords: Attenuated Salmonella; Enteric typhimurium; Oral DNA vaccine; Chickens.
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Nov 2013 02:41
Last Modified: 06 Oct 2015 00:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2012/264986
URI: http://psasir.upm.edu.my/id/eprint/25427
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