UPM Institutional Repository

Detection of Brucella melitensis in seropositive goats.


H., Plumeriastuti and Saad, Mohd Zamri (2012) Detection of Brucella melitensis in seropositive goats. Online Journal of Veterinary Research, 16 (1). pp. 1-7. ISSN 1328-925X


This study reports an association of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were re-collected from the 27 sero-positive goats 2 weeks later and subjected to RBPT for antibody titres. Uterii, mammary glands and supra-mammary lymph nodes were examined for pathological changes, immuno-peroxidase (IP) staining, bacterial isolation and PCR to detect B. melitensis. Results revealed that of the 27 goats tested, 41% were Category I (antibody titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4). Goats in Categories 2 and 3 revealed more severe pathological changes with the latter being most severe. All goats in Category 3 showed positive reaction to IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%) goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There was strong correlation between the antibody titre, pathological lesions and presence of B. melitensis.

Download File

PDF (Abstract)
Detection of Brucella melitensis in seropositive goats.pdf

Download (85kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Online Journal of Veterinary Research
Keywords: Brucella melitensis; Goats; Detection; Pathological changes.
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Nov 2013 03:13
Last Modified: 14 Sep 2015 00:07
URI: http://psasir.upm.edu.my/id/eprint/25417
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item