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Sea cucumber (Stichopus hermanii) based hydrogel to treat burn wounds in rats


Mohd Zohdi, Rozaini and Abu Bakar @ Zakaria, Md Zuki and Yusof, Norimah and Mohamed Mustapha, Noordin and Abdullah, Muhammad Nazrul Hakim (2011) Sea cucumber (Stichopus hermanii) based hydrogel to treat burn wounds in rats. Journal of Biomedical Materials Research: Part B Applied Biomaterials, 98B (1). pp. 30-37. ISSN 1552-4973; ESSN: 1552-4981


Malaysian sea cucumber was incorporated into hydrogel formulation by using electron beam irradiation technique and was introduced as novel cross-linked Gamat Hydrogel dressing. This study investigated whether Gamat Hydrogel enhanced repair of deep partial skin thickness burn wound in rats and its possible mechanism. Wounds were treated with either Gamat Hydrogel, control hydrogel, OpSite® film dressing or left untreated. Skin samples were taken at 7, 14, 21, and 28 days post burn for histological and molecular evaluations. Gamat Hydrogel markedly enhanced wound contraction and improved histological reorganization of the regenerating tissue. Furthermore, the dressing modulated the inflammatory responses, stimulated the activation and proliferation of fibroblasts, and enhanced rapid production of collagen fiber network with a consequently shorter healing time. The level of proinflammatory cytokines; IL-1α, IL-1β, and IL-6, were significantly reduced in Gamat Hydrogel treated wounds compared with other groups as assessed by reverse transcription-polymerase chain reaction (RT-PCR). In summary, our results showed that Gamat Hydrogel promoted burn wound repair via a complex mechanism involving stimulation of tissue regeneration and regulation of pro-inflammatory cytokines. The resultant wound healing effects were attributed to the synergistic effect of the hydrogel matrix and incorporated sea cucumber.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1002/jbm.b.31828
Publisher: Wiley Periodicals
Keywords: Burn; Cytokines; Hydrogel; Wound healing; Wound dressing
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Oct 2013 00:39
Last Modified: 16 Nov 2016 07:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jbm.b.31828
URI: http://psasir.upm.edu.my/id/eprint/25367
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