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Cryotop device enhances vitrification outcome of immature Bovine Oocytes.


Hajarian, Hadi and Haron, Wahid and Yusoff, Rosnina and Morteza, Daliri and Mojtaba, Dashtizad and Mirzapour, Tooba and Yimer, Nurhusien and Muhammad Modu, Bukar and Ismail, Mohd Iswadi and Othman, Abas Mazni (2011) Cryotop device enhances vitrification outcome of immature Bovine Oocytes. Journal of Animal and Veterinary Advances, 10 (19). pp. 2541-2545. ISSN 1680-5593


The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS), Electron Microscopy Grid (EMG) and cryotop for vitrification of immature bovine oocytes. Polar body, MII stage, survivability and subsequent developmental rates were compared. Only oocytes with 4-5 layers of cumulus cells were used. Oocytes were equilibrated in the first vitrification solution (VS1; HS+10% DMSO+10% Ethylene Glycol (EG)) for 30-45 sec and then in the second vitrification solution (VS2; 20% DMSO+20% EG+0.5 M Sucrose) for 25 sec. Within 30 sec they were mounted on one of the cryodevices and directly plunged into Liquid Nitrogen (LN2) for 10 days. Immature oocytes vitrified using cryotop represented higher rate of polar body extrusion and nuclear maturity (p<0.05). The highest survivability resulted from cryotop and EMG groups and no significant difference found between them. Vitrified oocytes in cryotop group had highest cleavage and blastocyst rates. All of the mean measured rates for vitrified/warmed immature oocytes were significantly lower than that of control group (p<0.05). In conclusion, results of this study showed the superiority of cryotop device for vitrification of immature bovine oocytes which resulted in higher viability and subsequent embryo development.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.3923/javaa.2011.2541.2545
Publisher: Medwell Publishing
Keywords: Vitrification; Cryodevice; Bovine; Immature oocytes; Eggs; Electron.
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Oct 2013 04:01
Last Modified: 14 Sep 2015 01:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/javaa.2011.2541.2545
URI: http://psasir.upm.edu.my/id/eprint/25354
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