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Histopathological features of peripheral T-cell lymphoma in Sprague Dawley rats induced with N-methyl-N-nitrosourea


Al-Salih, Hutheyfa A. H. and Hamzah, Hazilawati and Shaari, Mohd Rosly and Sabri, Jasni and Mohamed Mustapha, Noordin and Sithambaram, Shanmugavelu (2011) Histopathological features of peripheral T-cell lymphoma in Sprague Dawley rats induced with N-methyl-N-nitrosourea. Pertanika Journal of Tropical Agricultural Science, 34 (2). pp. 351-361. ISSN 1511-3701; ESSN: 2231-8542


This study described the histopathological features of peripheral T-cell lymphoma in male Sprague Dawley rats following intraperitoneal (i.p.) injections of N-methyl-N-nitrosourea (MNU) at a dose of 60 mg/kg body weight per injection, administered twice weekly for 2 consecutive weeks, and followed by a five-month’s observation period. Control rats were injected with normal saline, i.p. All the rats treated with MNU had enlargement of lymph nodes, with 30% had hepatosplenomegaly and 7% had enlarged kidneys at necropsy. Malignant lymphoma was observed in the lymph nodes, spleen, liver, lung, heart, and kidneys. The neoplastic cells were characterised as undifferentiated, and small to large size with bizarre pleomorphic nuclei. The severity was further described as mild, moderate and severe, based on the diffuseness of the lesions. Nonetheless, similar lesions were not observed in the thymus of the rats. Immunohistochemistry staining of the organs was positive for CD3 antibody, which is consistent with T-cell lymphoma.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Universiti Putra Malaysia Press
Keywords: N-methyl-N-nitrosourea; Rats; Peripheral T-cell lymphoma
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Oct 2013 07:34
Last Modified: 21 Nov 2016 07:43
URI: http://psasir.upm.edu.my/id/eprint/25342
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