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Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.


Citation

Moeini, Hassan and Abdul Rahim, Raha and Omar, Abdul Rahman and Yusoff, Khatijah (2011) Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. Comparative Immunology, Microbiology and Infectious Diseases, 34 (3). pp. 227-236. ISSN 0147-9571

Abstract

In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV.


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Official URL: http://www.elsevier.com/

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.cimid.2010.11.006
Publisher: Elsevier
Keywords: Chicken anemia virus; DNA vaccine; VP1; VP2.
Depositing User: Nur Farahin Ramli
Date Deposited: 23 Oct 2013 02:55
Last Modified: 16 Oct 2015 07:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.cimid.2010.11.006
URI: http://psasir.upm.edu.my/id/eprint/25331
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