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Anti-tumour promoting activity and antioxidant properties of girinimbine isolated from the stem bark of Murraya koenigii S.


Citation

Kok, Yih Yih and Lim, Yang Mooi and Ahmad, Kartini and Sukari, Mohd. Aspollah and Mat, Nashriyah and Rahmani, Mawardi and Ali, Abdul Manaf (2012) Anti-tumour promoting activity and antioxidant properties of girinimbine isolated from the stem bark of Murraya koenigii S. Molecules, 17 (4). pp. 4651-4660. ISSN 1420-3049

Abstract / Synopsis

Girinimbine, a carbazole alkaloid isolated from the stem bark of Murraya koenigii was tested for the in vitro anti-tumour promoting and antioxidant activities. Anti-tumour promoting activity was determined by assaying the capability of this compound to inhibit the expression of early antigen of Epstein-Barr virus (EA-EBV) in Raji cells that was induced by the tumour promoter, phorbol 12-myristate 13-acetate. The concentration of this compound that gave an inhibition rate at fifty percent was 6.0 μg/mL and was not cytotoxic to the cells. Immunoblotting analysis of the expression of EA-EBV showed that girinimbine was able to suppress restricted early antigen (EA-R). However, diffused early antigen (EA-D) was partially suppressed when used at 32.0 μg/mL. Girinimbine exhibited a very strong antioxidant activity as compared to α-tocopherol and was able to inhibit superoxide generation in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells more than 95%, when treated with the compound at 5.3 and 26.3 μg/mL, respectively. However girinimbine failed to scavenge the stable diphenyl picryl hydrazyl (DPPH)-free radical.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.3390/molecules17044651
Publisher: MDPI
Keywords: Murraya koenigii; Girinimbine; Anti-tumour promoting activity; Antioxidative; Superoxide.
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Nov 2013 09:11
Last Modified: 21 Sep 2015 08:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules17044651
URI: http://psasir.upm.edu.my/id/eprint/25292
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