UPM Institutional Repository

Liquid chromatography - tandem mass spectroscopic method for the determination Of zerumbone in human plasma and its application to Pharmacokinetics


Citation

Abdul, Ahmad Bustamam and Md. Sukari, Mohd. Aspollah and Abdullah, Rasedee and E. M. Eid, Eltayeb and O. Suliman, Fakhr Eldin and S. Fatah, Safa (2011) Liquid chromatography - tandem mass spectroscopic method for the determination Of zerumbone in human plasma and its application to Pharmacokinetics. Journal of Mass Spectrometry , 46 (8 ). pp. 772-781. ISSN 1076-5174; ESSN:1096-9888

Abstract

A rapid, sensitive, specific and selective LC-MS/MS method for the determination of zerumbone (ZER) in human plasma using 2,4-diamino-6-(4-methoxyphenyl)-1,3,5-triazine (DMTZ) as an internal standard (IS) has been developed and validated. ZER was chromatographed on C8 column using a mobile phase of acetonitrile/water (80:20, v/v) at a flow rate of 0.25 ml min(-1) . Quantitation was achieved using ESI+ interface, employing multiple reaction monitoring (MRM) mode at m/z 219 > 81 and 218 > 134 for ZER and IS, respectively. The calibration standards were linear over a range of 5-3000 ng ml(-1) (r(2)=0.9994) with an LLOQ of 5 ng ml(-1) (RSD %; 11.4% and bias%; 9.5%). Intra- and inter-day precision of ZER assay ranged from 0.18 to 3.56% with accuracy (bias) that varied between -5.09 and 4.3%, demonstrating good precision and accuracy. Recoveries of ZER and the IS from human plasma were above 85%. The developed method was validated for the determination of ZER in rat plasma. Linearity, stability of ZER and the ME on rat plasma were discussed. The applicability of the developed method was demonstrated by measuring ZER in rat plasma samples following intravenous and intraperitoneal administration of ZER prepared in hydroxypropyl-β-cyclodextrin (HPβCD) and sodium carboxymethyl cellulose (CMC), respectively, in 20 mg kg(-1) and this study indicated a clear significant difference (p<0.05) in pharmacokinetic parameters of ZER in ZER/HPβCD complex compared with ZER in CMC preparation.


Download File

[img]
Preview
PDF (Abstract)
Liquid chromatography.pdf

Download (188kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1002/jms.1942
Publisher: Wiley-Blackwell
Keywords: Tandem mass spectroscopic method; Zerumbone; Human plasma; Pharmacokinetics
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Aug 2013 04:48
Last Modified: 10 Feb 2016 06:44
URI: http://psasir.upm.edu.my/id/eprint/24902
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item