UPM Institutional Repository

Involvement of CYP450 system in hepatoprotective activity of Malaysian Agricultural Research and Development Institute (MARDI)-produced virgin coconut oils.


Citation

Zakaria, Zainul Amiruddin and Abdul Kadir, Arifah and Hakim Abdullah, Muhammad Nazrul and Ahmad, Zuraini and Rofiee, Mohd Salleh and Teh, Lay Kek and Salleh, Mohd Zaki and Long, Kamariah (2011) Involvement of CYP450 system in hepatoprotective activity of Malaysian Agricultural Research and Development Institute (MARDI)-produced virgin coconut oils. African Journal of Pharmacy and Pharmacology, 5 (23 ). pp. 2526-2536. ISSN 1996-0816

Abstract

The present study aims to determine the role of cytochrome P450 (CYP450) enzyme system in hepatoprotective activity of virgin coconut oils produced by Malaysian Agricultural Research and Development Institute (MARDI). Paracetamol (PCM)-induced hepatotoxic rat was used as a model. Liver injury induced by 3 g/kg PCM increased the liver weight and liver enzymes (e.g. alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphate (ALP)) and decreased cell viability indicating liver damage. Histological observation also confirms liver damage indicated by the presence of inflammations and necrosis. Pre-treatment with VCOA or VCOB reversed the significantly (P < 0.05) reversed PCM toxic effect. Groups pre-treated withvirgin coconut oil (VCOs) followed by inhibitor or inducer of CYP450 demonstrated significant (P < 0.0.5) increase in liver weight, liver enzymes levels and decrease in cell viability, which are, however, significantly (P < 0.05) less remarkable as compared to group treated with PCM alone. In conclusion, VCO possessed hepatoprotective effect, which is believed to be mediated via a non-CYP450 system and might be associated partly with the antioxidant potential of the oil. Further studies are warranted to determine the actual mechanisms of hepatoprotection involved.


Download File

[img]
Preview
PDF (Abstract)
Involvement of CYP450 system in hepatoprotective activity of Malaysian Agricultural Research and Development Institute.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.5897/AJPP11.021
Publisher: Academic Journals
Keywords: Malaysian Agricultural Research and Development Institute (MARDI); Virgin coconut oil; Livertoxicity; Paracetamol; Cytochrome P450; Hepatoprotection.
Depositing User: Nur Farahin Ramli
Date Deposited: 12 Sep 2013 04:49
Last Modified: 18 Nov 2015 03:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJPP11.021
URI: http://psasir.upm.edu.my/id/eprint/24727
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item