Simple Search:

Effect of protein concentration and injection pressure in microinjection delivery of maltose binding protein into breast cancer cells


Citation

Lim, Shen Ni and Allaudin, Zeenathul Nazariah and Mohd Lila, Mohd Azmi and Othman, Abas Mazni and Othman, Fauziah (2011) Effect of protein concentration and injection pressure in microinjection delivery of maltose binding protein into breast cancer cells. Pertanika Journal of Science & Technology, 19 (2). pp. 273-283. ISSN 0128-7680; ESSN: 2231-8526

Abstract / Synopsis

Microinjection is a powerful tool to deliver various substances, such as nucleic acids, proteins, peptides, RNA, and synthetic molecules into mammalian cells mechanically. Through microinjection, a controlled amount of protein can be delivered into the target cells to elucidate the specific functional effects in vitro. In this study, a series of protein microinjection optimization was performed in human breast cancer cells. The presence of Maltose Binding Protein (MBP) was microscopically monitored through indirect immunofluorescence assay. The optimization experimentation gave a high success rate when MBP protein was used at the minimum concentration of 1.5 mg/ml and at the injection pressures of 50 and 70 hPa. The average success rate of injections was 49.2±4.15% and 50.8±4.6%, while the average cell survivability was 50.98±4.67% and 49.72±5.48% at 50 and 70 hPa, respectively. The optimization of the MBP concentration and injection pressures successfully allowed an efficient delivery of precise protein dosage into breast cancer cells without any adverse effect. This microinjection optimization can be a practical guideline in any downstream applications of protein functional work.


Download File

[img]
Preview
PDF
#11 Pg 273-283.pdf

Download (1MB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Universiti Putra Malaysia Press
Keywords: Cell microinjection; Fluorescence microscope; Immunofluorescence; MBP
Depositing User: Nur Farahin Ramli
Date Deposited: 09 Sep 2013 12:17
Last Modified: 06 Jan 2016 09:12
URI: http://psasir.upm.edu.my/id/eprint/24689
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item