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Extended spectrum beta-lactamases among hospitalized patients in surgery wards, Ilam, Iran.


Sadeghifard, Nourkhoda and Ghafourian, Sobhan and Sekawi, Zamberi and Neela, Vasantha Kumari and Hematian, Ali and Pakzad, Iraj and Galehdari, Elham Abouali and Mohebi, Reza (2011) Extended spectrum beta-lactamases among hospitalized patients in surgery wards, Ilam, Iran. Journal of Microbial & Biochemical Technology, 3 (1). pp. 18-20. ISSN 1948-5948


Objectives of this study were to study the molecular epidemiology of ESBL-producing Klebsiella pneumoniae in selective hospitals in Iran, to determine the prevalence of TEM, SHV and CTX-M genes responsible for ESBL production among ESBL-producing K. pneumoniae, to investigate the susceptibility of Klebsiellae spp producing ESBLs towards non-beta-lactam antibiotics, all in different seasons. Clinical isolates of K. pneumoniae were identified during Mar. 2007 to Apr. 2008 in Ilam hospitals, the province in west of Iran. All isolates were found in surgery wards. ESBL activity was first evaluated using the standard disc diffusion test for cephalosporins and monobactam, then using the double-disc synergy test between cephalosporins and clavulanate. PCR assay had done for ESBLs genes detection. The results showed sixteen K.pneumoniae were identified by chemical methods. No resistance had occurred among K.pneumoniae toward non-beta-lactam antibiotics. BlaSHV was dominant gene responsible for ESBLs production while just one blaTEM along with blaSHV were found. BlaCTX-M was not responsible for ESBLs production in our study. 37.5% K.pneumoniae producing ESBLs in surgery ward in west of Iran must be considerable and need to further study in different part of hospital in Ilam and in Iran. Strict antibiotic policy should be adopted in hospitals to estimate the impact of higher resistance in bacteria and to take steps for reducing these resistances.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.4172/1948-5948.1000044
Publisher: OMICS Publishing
Keywords: Iran; K.pneumoniae; Surgery ward.
Depositing User: Nur Farahin Ramli
Date Deposited: 18 Mar 2014 07:46
Last Modified: 18 Mar 2014 07:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4172/1948-5948.1000044
URI: http://psasir.upm.edu.my/id/eprint/24572
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