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Isolation and cytotoxicity of triterpenes from the roots of Phyllanthus pulcher Wall. ex Müll. Arg. (Euphorbiaceae).


Citation

Bagalkotkar, Gururaj and Tang, Seng Chuan and Khalivulla, Shaik Ibrahim and Hamzah, Ahmad Sazali and Shaari, Khozirah and Lajis, Md. Nordin and Saad, Mohammad Said and Stanslas, Johnson (2011) Isolation and cytotoxicity of triterpenes from the roots of Phyllanthus pulcher Wall. ex Müll. Arg. (Euphorbiaceae). African Journal of Pharmacy and Pharmacology, 5 (2). pp. 183-188. ISSN 1996-0816

Abstract

The dried powdered roots of Phyllanthus pulcher Wall. ex Müll. Arg. (Euphorbiaceae), were sequentially extracted with dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH). The extracts were tested for cytotoxic activity against three human cancer cell lines: MCF-7 (breast), NCI-H460 (lung) and DU-145 (prostate). The DCM extract exhibited the strongest cytotoxic activity compared with EtOAc and MeOH extracts. Hence from the DCM extract, five pentacyclic triterpenes, 3α-acetoxyl-25-hydroxyolean-12-en-28-oic acid (1), glochidone (2), 12(13)-dehydro-3α-acetoxyolean-28-oic acid (3), lupanyl acetate (4) and glochidonol (5) were isolated and identified by spectroscopic analyses (1H NMR, 13C NMR, FT-IR, UV, DEPT, HMQC, HMBC and HREIMS). This is the first report on the isolation of 4 from a natural source, whereas 1 and 3 have already been isolated from the families Hamamelidaceae and Compositae (Asteraceae), respectively. However this is the first study reporting the presence of 1 and 3 in the Euphorbiaceae family. The isolated tritepenes 1-5 were tested against the three human tumour cell lines as stated above. Only compounds 1 and 5 exhibited cytotoxic activity, 5 being most potent with IC50 values ranging 7.5–13.4 µg/mL (17.1–30.5 µM).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.5897/AJMR10.331
Publisher: Academic Journals
Keywords: Phyllanthus pulcher; Triterpenoids; Cytotoxic activity; Breast cancer; Lung cancer; Prostate cancer.
Depositing User: Nur Farahin Ramli
Date Deposited: 26 Mar 2014 00:15
Last Modified: 26 Aug 2015 07:29
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJMR10.331
URI: http://psasir.upm.edu.my/id/eprint/24538
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