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Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level of moisture condition through statistical optimization.


Citation

Kamal Bahrin, Ezyana and Ibrahim, Mohamad Faizla and Abdul Razak, Mohd Nafis and Abd. Aziz, Suraini and Md. Shah, Umi Kalsom and Mohamed Alitheen, Noorjahan Banu and Md Salleh, Madihah (2012) Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level of moisture condition through statistical optimization. Reparative Biochemistry & Biotechnology , 42 (2). pp. 155-170. ISSN 1082-6068

Abstract

The response surface method was applied in this study to improve cellulase production from oil palm empty fruit bunch (OPEFB) by Botryosphaeria rhodina. An experimental design based on a two-level factorial was employed to screen the significant environmental factors for cellulase production. The locally isolated fungus Botryosphaeria rhodina was cultivated on OPEFB under solid-state fermentation (SSF). From the analysis of variance (ANOVA), the initial moisture content, amount of substrate, and initial pH of nutrient supplied in the SSF system significantly influenced cellulase production. Then the optimization of the variables was done using the response surface method according to central composite design (CCD). Botryosphaeria rhodina exhibited its best performance with a high predicted value of FPase enzyme production (17.95U/g) when the initial moisture content was at 24.32%, initial pH of nutrient was 5.96, and 3.98g of substrate was present. The statistical optimization from actual experiment resulted in a significant increment of FPase production from 3.26 to 17.91U/g (5.49-fold). High cellulase production at low moisture content is a very rare condition for fungi cultured in solid-state fermentation.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1080/10826068.2011.585413
Publisher: Taylor & Francis
Keywords: Cellulase; Optimization; Response surface methodology; Solid-state fermentation.
Depositing User: Nur Farahin Ramli
Date Deposited: 08 Oct 2013 09:16
Last Modified: 21 Sep 2015 00:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10826068.2011.585413
URI: http://psasir.upm.edu.my/id/eprint/24212
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