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A quick DNA extraction protocol: without liquid nitrogen in ambient temperature


Citation

Ferdous, Jannatul and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Syed Muhammad, Sharifah Kharidah (2012) A quick DNA extraction protocol: without liquid nitrogen in ambient temperature. African Journal of Biotechnology, 11 (27). art. no. 197554430636. pp. 6956-6964. ISSN 1684–5315

Abstract / Synopsis

Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Food Science and Technology
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.5897/AJB11.3283
Publisher: Academic Journals
Keywords: Different plant tissues; Inexpensive; Rice flour; Rapid DNA extraction
Depositing User: Nur Farahin Ramli
Date Deposited: 10 Oct 2013 11:00
Last Modified: 09 Nov 2017 17:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB11.3283
URI: http://psasir.upm.edu.my/id/eprint/24185
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