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Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on chemometry of 1H nuclear magnetic resonance spectroscopy


Citation

Maulidiani, and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Shitan, Mahendran and Kneer, Ralf and Neto, Victor and Lajis, Md. Nordin (2012) Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on chemometry of 1H nuclear magnetic resonance spectroscopy. Journal of Agricultural and Food Chemistry, 60 (1). pp. 410-417. ISSN 0021-8561; ESSN: 1520-5118

Abstract / Synopsis

The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing (1)H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of (1)H NMR spectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Forestry
Faculty of Science
Institute for Mathematical Research
Institute of Bioscience
DOI Number: https://doi.org/10.1021/jf200270y
Publisher: American Chemical Society
Keywords: Pegaga varieties; Growth-lighting effects; Metabolites content; Chemometry; 1H nuclear magnetic resonance
Depositing User: Nur Farahin Ramli
Date Deposited: 18 Oct 2013 12:26
Last Modified: 28 Apr 2016 16:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1021/jf200270y
URI: http://psasir.upm.edu.my/id/eprint/24147
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