UPM Institutional Repository

Occurrence of Norovirus GI in green and red onion


Citation

Mohd Sahroni, Noor Hidayah and Tuan Chilek, Tuan Zainazor and Pui, Chai Fung and Ahmad, Noorlis and Mat Radzi, Noor Eliza and Mohamed Dasuki, Naziehah and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2011) Occurrence of Norovirus GI in green and red onion. International Food Research Journal, 18 (2). pp. 677-681. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Several Norovirus cases due to consumption of green onions have been reported during recent years but reports on red onions are not found. Onions are one of the major tastes in Malaysian food which are sometimes consuming raw especially the green onion. Viral contamination in onions can occur due to planting condition and not properly prepared food. This situation can pose the human health risk. A method was developed to detect the Norovirus that might present on different type of onions. In this study, 60 samples were collected from local market. Elution by Tryptose Phosphate Glycine broth and concentration steps using negatively charge filter were applied to enhance the detection of virus in food due to low copies of virus on food surface. The viral RNA was extracted using Qiagen Rneasy Mini kit before further detection using One-step RT-PCR. The total incidence of Norovirus in green onion and red onion was 13.33% (4/30) and 3.33 % (1/30) respectively. This is the first report of the detection of Norovirus in red and green onions in Malaysia. Based on the results, it is concluded that this method is reliable to detect Norovirus on onions and vegetables surface and hence can be applied in the laboratories for routine or food borne outbreak investigation.


Download File

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Green onion; Negative charge filter; Norovirus; One-step RT-PCR; Red onion
Depositing User: Nur Farahin Ramli
Date Deposited: 19 Feb 2014 07:37
Last Modified: 22 Nov 2019 00:46
URI: http://psasir.upm.edu.my/id/eprint/24074
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item