UPM Institutional Repository

Growth and survival of lactic acid bacteria isolated from byproduct of virgin coconut oil as probiotic candidate for poultry.


Citation

Husmaini, and M. H., Abbas and E., Purwati and A., Yuniza and Alimon, Abdul Razak (2011) Growth and survival of lactic acid bacteria isolated from byproduct of virgin coconut oil as probiotic candidate for poultry. International Journal of Poultry Science, 10 (4). pp. 309-314. ISSN 1682-8356; ESSN:1994-7992

Abstract

The main objective of this research is to investigate the potential of LABs isolated from processing byproduct of the VCO in in vitro conditions for use as probiotics in poultry. Forty eight LABs were isolated and four of them have been selected for further study i.e. Lh1, Lh2, Lh3 and Lh4. A series of tests carried out by studying the ability of bacteria to survive at 37 and 42°C, tolerance of LAB at pH 2, 0, 5, 7 and 7, 0 and tolerance to gastric juice as well as sensitivity to several antibiotics commonly was given to poultry. The survival of LABs was evaluated after 15, 30, 60, 90 and 120 and 300 min of incubation. The sensitivity test to antibiotics was performed by Muller Hinton's agar. All the bacteria showed tolerance and ability to grow at pH 5 and 7, but only Lh4 enabled to tolerate at pH 2. All of LAB can grow at gastric juice stimulated. Lh4 was not sensitive to all antibiotics (clear zones: 0.33 mm) but the other LABs were sensitive (clear zones: 5-12 mm). The conclusion of this research is the ability of LABs to grow in in vitro conditions varies. The Lh4 has demonstrated its ability to grow and the best survival with the OD (λ = 580) is 1.99 after 300 min of incubation at pH 2 and has shown the most resistant to all antibiotics tested with a wide clear zone 0.33 mm, hence potentially be used for probiotic in poultry.


Download File

[img]
Preview
PDF (Abstract)
Growth and survival of lactic acid bacteria isolated from byproduct of virgin coconut oil as probiotic candidate for poultry.pdf

Download (185kB) | Preview
Official URL or Download Paper: http://www.ansinet.com/

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.3923/ijps.2011.309.314
Publisher: Asian Network for Scientific Information (ANSINET)
Keywords: Lactic acid bacteria; Byproduct VCO; Survival; Growth; Probiotic.
Depositing User: Nur Farahin Ramli
Date Deposited: 27 Nov 2013 07:47
Last Modified: 14 Sep 2015 02:41
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ijps.2011.309.314
URI: http://psasir.upm.edu.my/id/eprint/23824
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item