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In vitro performances of hypocotyl and cotyledon explants of tomato cultivars under sodium chloride stress


Citation

Mohamed, Abdalmajid Nasher and Ismail, Mohd Razi and Abdul Kadir, Mihdzar and Mohd Saud, Halimi (2011) In vitro performances of hypocotyl and cotyledon explants of tomato cultivars under sodium chloride stress. African Journal of Biotechnology, 10 (44). art. no. F972C3B30037. pp. 8757-8764. ISSN 1684–5315

Abstract

A plant tissue culture technique is a good method for the evaluation and screening of plant genotypes for salt tolerance. In vitro evaluations of sodium chloride (NaCl) effects on two tomato cultivars (Pearl and Beril) were investigated with four NaCl levels (0, 25, 50 and 75 mM) using hypocotyl and cotyledon explants. The explants were cultured in MS media having 2.0 mg/l BAP along with different concentrations of NaCl. Sodium chloride stress negatively affected the growth traits and chlorophyll content. Significant differences were noticed between the cultivars followed by different NaCl levels, where the Beril responded superior than that of Pearl. The type of explant showed a difference in their response to shoots regeneration under NaCl stress, where the cotyledon explants achieved best results than hypocotyl explants.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
Publisher: Academic Journals
Keywords: Cotyledons; Hypocotyls; In vitro; Salt stress; Tomato
Depositing User: Nur Farahin Ramli
Date Deposited: 31 Jan 2014 01:02
Last Modified: 15 Nov 2017 02:49
URI: http://psasir.upm.edu.my/id/eprint/23776
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