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Purification of rabbit polyclonal immunoglobulin G using anion exchangers.


Tey, Beng Ti and Taip, Farah Saleena and Tan, Wen Siang and Ling, Tau Chuan and Wongchuphan, Rattana and Subramaniam, Senthil Kumar (2011) Purification of rabbit polyclonal immunoglobulin G using anion exchangers. Process Biochemistry, 46 (Januar). pp. 101-107. ISSN 1359-5113


Negative chromatography antibody purification (N-CAP) using the weak anion exchanger STREAMLINE™ DEAE to extract impurities while retaining the target antibody is proposed as an effective method for the recovery of antibody from rabbit serum. The effects of pH and initial protein concentration on the removal of albumin were investigated. The optimal pH and initial protein concentration for the efficient removal of albumin from rabbit serum were pH 8.0 and 0.5 mg/ml, respectively. Under optimal binding conditions, DEAE successfully removed more than 90% of the albumin from rabbit serum with less than 20% IgG loss. This process offered good polyclonal IgG yield of 80% with a purity of 83% and a purification factor of 5.5. The use of a strong anion exchanger like STREAMLINE™ Q XL for albumin removal was also explored. Under similar optimized conditions, albumin removal by Q XL was as high as 90%. However, IgG recovery and purity were reduced to about 70% and 62%, respectively. Thus, N-CAP using the anion exchanger DEAE removes albumin from rabbit serum and thereby offers an efficient means of purifying polyclonal antibodies.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.1016/j.procbio.2010.07.023
Keywords: Polyclonal IgG; STREAMLINE™ DEAE; Rabbit serum; Albumin removal; Anion exchange adsorbents; Negative chromatography antibody purification
Depositing User: Muizzudin Kaspol
Date Deposited: 09 Sep 2014 08:10
Last Modified: 12 Jan 2016 04:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2010.07.023
URI: http://psasir.upm.edu.my/id/eprint/23385
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