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Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent


Citation

Abdul Razak, Che Nyonya and Salleh, Abu Bakar and Musani, Rosiah and Abd. Samad, Mohd. Yusoff and Basri, Mahiran (1997) Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent. Journal of Molecular Catalysis B: Enzymatic, 3 (1-4). pp. 153-159. ISSN 1381-1177; ESSN: 1873-3158

Abstract / Synopsis

Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH and temperature for both lipases were 6.0 and 45°C, respectively. However, lipase from R. rhizopodiformis was slightly more thermostable than that of R. oryzae lipase but the latter was more stable over broader pH ranges compared to the former, specially at acidic pH. Both the enzyme showed rapid loss of activities at temperatures above 50°C and pH above 7.0. Lipase from R. oryzae showed broader specificities to substrates than R. rhizopodiformis, but both displayed little reactivity for triacetin and polyoxysorbitan (Tween 20, 40, and 60). Both the lipases are 1,3 specific.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1016/S1381-1177(96)00035-5
Publisher: Elsevier
Keywords: Lipases; Rhizopus oryzae; R. rhizopodiformis; Thermophilic fungi
Depositing User: Nabilah Mustapa
Date Deposited: 02 Feb 2016 12:06
Last Modified: 02 Feb 2016 12:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S1381-1177(96)00035-5
URI: http://psasir.upm.edu.my/id/eprint/22567
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