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A plate assay for primary screening of lipase activity


Abd Samad, Mohd Yusoff and Abdul Razak, Che Nyonya and Salleh, Abu Bakar and Wan Yunus, Wan Md. Zin and Ampon, Kamaruzaman and Basri, Mahiran (1989) A plate assay for primary screening of lipase activity. Journal of Microbiological Methods, 9 (1). pp. 51-56. ISSN 0167-7012; ESSN: 1872-8359


A method for primary plate assay to determine lipase activity was developed. Tween 80 was used as the substrate with either Victoria Blue B, methyl red or rhodamine B as the indicator. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24 h. Similar results were obtained using Tween 20 and 60 as substrates. Intensity of the colour is greater than that of the trioleindye system and clearer than the hydrolysis zone of tributyrin plate. Tests using a commercial enzyme preparation and growth media with lipolytic activity showed that the zone of intensification increased with increased lipolytic activity. A linear relationship can be seen when log enzyme concentration is plotted against the diameter of zone of intensification. Using this technique, primary screening of lipolytic microorganisms can be conducted using the formation of zones of intensification around the colonies and mycelia.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1016/0167-7012(89)90030-4
Publisher: Elsevier
Keywords: Plate assay; Lipase; Tween
Depositing User: Nabilah Mustapa
Date Deposited: 02 Feb 2016 04:06
Last Modified: 02 Feb 2016 04:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/0167-7012(89)90030-4
URI: http://psasir.upm.edu.my/id/eprint/22566
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