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Improvement of Aspergillus flavus Link S44-1 using random mutational method for kojic acid production


Prabu, Rajagopalan and Mohamad, Rosfarizan and Md. Shah, Umi Kalsom and Ariff, Arbakariya (2011) Improvement of Aspergillus flavus Link S44-1 using random mutational method for kojic acid production. Minerva Biotechnologica, 23 (4). pp. 83-91. ISSN 1120-4826; ESSN: 1827-160X


AIM: The aim of this study was to improve the kojic acid producing strain, A. flavus Link S44-1, by random mutation methods. METHODS: Random induced mutation method was applied to A. flavus Link S44-1 with N-Methyl-N’ nitoro-N nitrosoguandine (NTG), ultraviolet (UV), and γ irradiation for improvement of its ability in producing kojic acid. The changes in the activity of cell bound enzyme responsible for kojic acid synthesis after the mutation were evaluated. RESULTS: The improved mutants (A. flavus NTG-MTDC-22, A. flavus UV-MTDC-12, and A. flavus G-MTDC-8) were capable of producing kojic acid up to final concentrations of 46, 42, and 49 g/L in fed-batch fermentation respectively. These which were 2.3 to 2.7 fold higher than the parent strain. In the mutated strains, the activities of enzymes related to kojic acid pathway such as glucose dehydrogenase and gluconate dehydrogenase detected during the fermentation were significantly higher than the parent strain. Spores of the mutants were stable and have consistent ability in producing kojic acid after prolonged storage in glycerol at -20 °C. CONCLUSION: The mutant A. flavus and the simple method used to improve the strains have potential applications in the development of fermentation process for industrial production of kojic acid, where the demand for this organic acid has increased enormously with its increasing applications in various industries.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Edizioni Minerva Medica
Keywords: Aspergillus flavus; Glucose dehydrogenase; Methylnitronitrosoguanidine; Ultraviolet rays
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 02 Sep 2015 00:01
Last Modified: 28 Oct 2015 06:47
URI: http://psasir.upm.edu.my/id/eprint/22415
Statistic Details: View Download Statistic

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