UPM Institutional Repository

Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus


Shahriarinour, Mahdi and Abdul Wahab, Mohd Noor and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Ariff, Arbakariya (2011) Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus. African Journal of Biotechnology, 10 (38). art. no. 2C68E2D33210. pp. 7459-7467. ISSN 1684–5315


The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified OPEFB fiber. In fermentation with the addition of Tween 80 (2 ml L-1) as surfactant, the production of cellulase was increased by two-fold as compared to fermentation without surfactant. Cellulase production by A. terreus was also enhanced with the addition of calcium chloride (3 mM) and magnesium sulfate (5 mM) in the medium. Optimum pH and temperature for cellulase production by A. terreus was 5.5 and 28°C, respectively. Cellulase production in agitated shake flask fermentation at 200 rpm was four times higher as compared to fermentation in static flask.

Download File

[img] PDF
Effect of medium composition and cultural condition on.pdf
Restricted to Repository staff only

Download (160kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Academic Journals
Keywords: Optimization; Aspergillus terreus; Submerged fermentation; Cellulase; Oil palm empty fruit bunch
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 01 Sep 2015 09:02
Last Modified: 14 Nov 2017 02:03
URI: http://psasir.upm.edu.my/id/eprint/22413
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item