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Reduction of molybdate to molybdenum blue by Klebsiella sp. strain hkeem


Lim, H. K. and Syed, Mohd. Arif and Abd. Shukor, Mohd. Yunus (2012) Reduction of molybdate to molybdenum blue by Klebsiella sp. strain hkeem. Journal of Basic Microbiology, 52 (3). pp. 296-305. ISSN 0233-111X; ESSN: 1521-4028


A novel molybdate-reducing bacterium, tentatively identified as Klebsiella sp. strain hkeem and based on partial 16s rDNA gene sequencing and phylogenetic analysis, has been isolated. Strain hkeem produced 3 times more molybdenum blue than Serratia sp. strain Dr.Y8; the most potent Mo-reducing bacterium isolated to date. Molybdate was optimally reduced to molybdenum blue using 4.5 mM phosphate, 80 mM molybdate and using 1% (w/v) fructose as a carbon source. Molybdate reduction was optimum at 30 °C and at pH 7.3. The molybdenum blue produced from cellular reduction exhibited absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Inhibitors of electron transport system such as antimycin A, rotenone, sodium azide, and potassium cyanide did not inhibit the molybdenum-reducing enzyme. Mercury, silver, and copper at 1 ppm inhibited molybdenum blue formation in whole cells of strain hkeem.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1002/jobm.201100121
Publisher: Wiley
Keywords: Klebsiella sp. strain hkeem; Molybdate reduction; Molybdenum blue
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 07 Dec 2015 06:13
Last Modified: 07 Dec 2015 06:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jobm.201100121
URI: http://psasir.upm.edu.my/id/eprint/22394
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