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A partial length cyclophilin-encoding (cyp) cDNA isolated from Ganoderma boninense


Lim, Fook Hwa and Iskandar, Nor Fakhrana and Shaharuddin, Noor Azmi and Abd Rasid, Omar and Abu Seman, Idris and Ghulam Ahmad, Ahmad Parveez (2011) A partial length cyclophilin-encoding (cyp) cDNA isolated from Ganoderma boninense. Journal of Oil Palm Research, 23. pp. 1115-1120. ISSN 1511-2780


Oil palm is sub­jected to var­i­ous pests and dis­eases, one of the most impor­tant being basal stem rot (BSR) dis­ease which is caused by a few species of Gan­o­derma. Gan­o­derma is a white rot fun­gus that attacks oil palm around the world. Dur­ing plant infec­tion, a few genes are highly expressed in the fun­gus and have been linked to fun­gal path­o­genic­ity. One of these genes is the cyclophilin-encoding gene (cyp). In this study, degen­er­ate primers have been designed based on the con­served regions of the gene. PCR ampli­fi­ca­tion suc­cess­fully ampli­fied a 212 bp DNA frag­ment from Gan­o­derma boni­nense cDNAs. The DNA sequence analy­sis showed that the frag­ment has about 80% sim­i­lar­ity to other plant path­o­genic fun­gal cyp genes. The trans­lated amino acid sequence was also highly iden­ti­cal to other plant path­o­genic fun­gal cyclophilin. Con­serve domain search results show that the obtained sequence has sim­i­lar­ity to the cyclophilin_ABH-like domain.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Malaysian Palm Oil Board
Keywords: Basal stem rot disease; cDna; Cyclophilin; Fungal pathogenicity; Ganoderma boninense
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 07 Dec 2015 05:22
Last Modified: 07 Dec 2015 05:22
URI: http://psasir.upm.edu.my/id/eprint/22388
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