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Flavonoids production in Hydrocotyle bonariensis callus tissues


Masoumian, M. and Ariff, Arbakariya and Ahmad, Syahida and Mahmood, Maziah (2011) Flavonoids production in Hydrocotyle bonariensis callus tissues. Journal of Medicinal Plants Research, 5 (9). pp. 1564-1574. ISSN 1996-0875


Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production was in DKW basal medium. Fructose as a carbon source increased the flavonoids content by 3%. Callus treated with 2 mg/L 2,4-D in comparison with the other auxins and among the cytokinins, kinetin and thidiazuron (TDZ) at 1 mg/L enhanced the flavonoid accumulation. Higher flavonoid production was obtained in combination with the 2 mg/L 2,4-D and 1 mg/L kinetin, while the pH value which was higher than 5.7 showed inhibitory effect on flavonoids production. It was found that the highest flavonoids were produced at 16 days of culture.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Academic Journals
Keywords: Flavonoids; Cell culture; Media composition
Depositing User: Nabilah Mustapa
Date Deposited: 25 Sep 2015 07:48
Last Modified: 16 Oct 2015 07:16
URI: http://psasir.upm.edu.my/id/eprint/22376
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