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Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues


Masoumian, M. and Ariff, Arbakariya and Ahmad, Syahida and Mahmood, Maziah (2011) Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues. African Journal of Biotechnology, 10 (32). art. no. CB9F21230655. pp. 6021-6029. ISSN 1684–5315


Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Academic Journals
Keywords: Flavonoids; Cell culture; Amino acid; Precursor
Depositing User: Nabilah Mustapa
Date Deposited: 25 Sep 2015 07:53
Last Modified: 15 Nov 2017 03:11
URI: http://psasir.upm.edu.my/id/eprint/22375
Statistic Details: View Download Statistic

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