Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope

Citation

Mumtaz, Tabassum and Abd. Aziz, Suraini and Abdul Rahman, Nor'aini and Phang, Lai Yee and Wasoh @ Mohamad Isa, Helmi and Shirai, Yoshihito and Hassan, Mohd Ali (2011) Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope. International Journal of Polymer Analysis and Characterization, 16 (4). pp. 228-238. ISSN 1023-666X; ESSN: 1563-5341

Abstract

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) granule formation in vivo in wild type Comamonas sp. EB172 grown in mixed organic acids under nitrogen limitation were visualized under transmission electron microscope (TEM). The early stages of PHBV production revealed dark-stained mediation elements near the center of the cell supporting the third model of granule formation. The native granules revealed characteristic core-shell structure in which white PHB cores were surrounded by darker PHBV shells. In vitro, the copolymer showed bimodal molecular weight distribution and exhibited two melting peaks in the differential scanning calorimeter (DSC) thermogram, supporting the formation of block copolymer.

Download File

Preview

PDF ( Abstract)
Viscous flow due to a permeable stretching.pdf
Download (180kB) | Preview

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences
DOI Number:	https://doi.org/10.1080/1023666X.2011.569990
Publisher:	Taylor & Francis
Keywords:	Comamonas sp.; 8B172; PHBV granules; TEM
Depositing User:	Nurul Ainie Mokhtar
Date Deposited:	01 Sep 2015 04:46
Last Modified:	02 Sep 2016 08:10
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/1023666X.2011.569990
URI:	http://psasir.upm.edu.my/id/eprint/22355
Statistic Details:	View Download Statistic

Actions (login required)

View Item