UPM Institutional Repository

Basic characteristics of cellulase immobilized on lignophenol


Nonaka, Hiroshi and Ariffin, Hidayah and Funaoka, Masamitsu (2011) Basic characteristics of cellulase immobilized on lignophenol. Kobunshi Ronbunshu, 68 (5). pp. 315-319. ISSN 0386-2186


Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable.

Download File

PDF (Abstract )
Basic characteristics of cellulase immobilized on lignophenol.pdf

Download (180kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1295/koron.68.315
Publisher: Nihon Kobunshi Gakkai,Society of Polymer Science, Japan
Keywords: Cellulase; Lignophenol; Immobilized Enzyme; Physical adsorption; Enzymatic hydrolysis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 01 Sep 2015 04:06
Last Modified: 10 Dec 2015 03:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1295/koron.68.315
URI: http://psasir.upm.edu.my/id/eprint/22349
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item