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Production of long helical capsid of Nipah virus by Pichia pastoris.


Joseph, Narcisse M. S. and Tey, Beng Ti and Tan, Chon Seng and Shafee, Norazizah and Tan, Wen Siang (2011) Production of long helical capsid of Nipah virus by Pichia pastoris. Process Biochemistry, 46 (9). pp. 1871-1874. ISSN 1359-5113; ESSN: 1873-3298


The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5 μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.procbio.2011.06.004
Publisher: Elsevier
Keywords: Paramyxovirus; Nipah virus; Nucleocapsid protein; Helical structure; Pichia pastoris.
Depositing User: Najwani Amir Sariffudin
Date Deposited: 10 Jul 2014 00:41
Last Modified: 06 Oct 2015 02:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2011.06.004
URI: http://psasir.upm.edu.my/id/eprint/22301
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