Citation
Abstract
In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), VR of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering Faculty of Science |
| DOI Number: | https://doi.org/10.1016/j.seppur.2011.07.039 |
| Publisher: | Elsevier |
| Keywords: | Aqueous two phase system; Cyclodextrin glycosyltransferase; Cyclodextrin; Enzyme; Purification. |
| Depositing User: | Najwani Amir Sariffudin |
| Date Deposited: | 10 Jul 2014 00:15 |
| Last Modified: | 10 Dec 2015 02:03 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.seppur.2011.07.039 |
| URI: | http://psasir.upm.edu.my/id/eprint/22298 |
| Statistic Details: | View Download Statistic |
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