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Effect of oil palm empty fruit bunch (OPEFB) particle size on cellulase production by Botryosphaeria sp. in solid state fermentation.


Citation

Kamal Bahrin, Ezyana and Piong, Yeau Seng and Abd. Aziz, Suraini (2011) Effect of oil palm empty fruit bunch (OPEFB) particle size on cellulase production by Botryosphaeria sp. in solid state fermentation. Australian Journal of Basic and Applied Sciences, 5 (3). pp. 276-280. ISSN 1991-8178

Abstract

Locally isolated Botryosphaeria sp. showed the ablity to produce cellulases (FPase, CMCase and β-glucosidase) from oil palm empty fruit bunch (OPEFB) as substrate. Different particle sizes (0.25-0.3 mm, 0.42-0.6 mm, 0.84-1.0 mm and 5.0-10 mm) of OPEFB were investigated under solid state fermentation on the cellulase production. The highest production of FPase and β-glucosidase were obtained from OPEFB particle size of 0.42 - 0.60 mm with 3.261 ± 0.011 U/g and 0.115 ± 0.008 U/g, respectively. It was found that among the four different OPEFB particle sizes studied, particle size of 0.84 - 1.0 mm gave the highest activity of CMCase (8.134 ± 0.071 U/g). Highest concentration of reducing sugars produced in this experiment was 4.303 ± 0.095 mg/ml.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: INSInet Publication
Keywords: Substrate particle size; Cellulase; Solid state fermentation; Botryosphaeria sp..
Depositing User: Nur Farahin Ramli
Date Deposited: 02 Jan 2014 04:04
Last Modified: 21 Sep 2015 08:13
URI: http://psasir.upm.edu.my/id/eprint/22286
Statistic Details: View Download Statistic

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