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Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator.


Citation

Siddiquee, Shafiquzzaman and Yusof, Nor Azah and Salleh, Abu Bakar and Tan, Soon Guan and Abu Bakar, Fatimah (2011) Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. Microchimica Acta, 172 (3-4). pp. 357-363. ISSN 0026-3672; ESSN:1436-5073

Abstract

An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10-14 to 1.8 × 10-4 mol L-1, with a detection limit of 1.0 × 10-15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s00604-010-0498-7
Publisher: Springer Verlag
Keywords: Chitosan; DNA electrochemical biosensor; DNA hybridization.
Depositing User: Nur Farahin Ramli
Date Deposited: 02 Jan 2014 03:52
Last Modified: 18 Feb 2016 04:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s00604-010-0498-7
URI: http://psasir.upm.edu.my/id/eprint/22280
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