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Regeneration capacity of cell suspension culture in Malaysian rice genotypes under salinity stress


Htwe, Nwe Nwe and Mahmood, Maziah and Ho, Chai Ling and Qamaruz Zaman, Faridah and Md Zain, Abdullah (2011) Regeneration capacity of cell suspension culture in Malaysian rice genotypes under salinity stress. Asian Journal of Biotechnology, 3 (4). pp. 357-367. ISSN 1996-0700


Rice, a salt-sensitive species has a substantial genetic variation for salt tolerance within the cultivated gene pool. This study describes the establishment of cell suspension culture system and analysis of proline content of the selected rice genotypes under in vitro salt stress. The cell suspension cultures, initiated from the friable seed-derived callus, were established in MS medium supplemented with different concentrations of 2,4-D (0-25 μM) and subjected to different concentrations of NaCl (0-250 mM). The average Settled Cell Volume (SCV) of different clumps sizes was recorded. The results showed that MR219-4 exhibited the highest cell growth rate and regeneration capacity. The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size. The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP. However, low regeneration capacity was obtained in NaCl treatments. MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4. In addition, all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation and MR219-4 revealed the lowest content among genotypes studied. These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress. This finding also suggests that proline may play a crucial role in protecting rice cells under salinity stress.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.3923/ajbkr.2011.357.367
Publisher: Knowledgia Review, Malaysia
Keywords: Cell suspension; In vitro studies; Rice (Oryza sativa); Shoot regeneration; Salinity stress; Proline
Depositing User: Najwani Amir Sariffudin
Date Deposited: 09 Jul 2014 03:51
Last Modified: 27 Oct 2017 04:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajbkr.2011.357.367
URI: http://psasir.upm.edu.my/id/eprint/22276
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