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Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man.


Citation

Saedi, Tayebeh Azam and Moeni, Hassan and Tan, Wen Siang and Yusoff, Khatijah and Mohd. Daud, Hassan and Chu, Kua Beng and Tan, Soon Guan and Bhassu, Subha (2012) Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. Molecular Biology Reports, 39 (5). pp. 5785-5790. ISSN 0301-4851; ESSN: 1573-4978

Abstract

White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M.rosenbergii cultured in Malaysia. In the present study,RT-PCR assay was used to detect the causative agents of WTD, M.rosenbergii nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples.Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from the other isolates. Histopathological studies revealed myofiber degeneration of the tail muscles and liquefactive myopathy in the infected prawns. This was the first report on the occurrence of MrNV in the Malaysian freshwater prawn.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1007/s11033-011-1389-7
Publisher: Springer
Keywords: Macrobrachium rosenbergii; Nodavirus; White tail disease; RT-PCR; Histopathology.
Depositing User: Najwani Amir Sariffudin
Date Deposited: 17 Jun 2014 01:54
Last Modified: 17 Feb 2016 02:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11033-011-1389-7
URI: http://psasir.upm.edu.my/id/eprint/22234
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