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Cyanobacterial biomass as n-supplement to oil palm empty fruit bunch (opefb) fibre for improvement of cellulase production by Aspergillus terreus in submerged fermentation.


Citation

Shariarinour, Mahdi and Abd Wahab, Mohd. Noor and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Ariff , Arbakariya (2011) Cyanobacterial biomass as n-supplement to oil palm empty fruit bunch (opefb) fibre for improvement of cellulase production by Aspergillus terreus in submerged fermentation. Bioresources, 6 (2). pp. 1696-1706. ISSN 1930-2126; ESSN: 1930-2126

Abstract

The possibility of using dry biomass of a cyanobacterium, Anacystis nidulans, as nitrogen source supplement for improvement of cellulase production by Aspergillus terreus was studied in submerged fermentation using oil palm empty fruit bunch (OPEFB) fibre as a carbon source. For comparison, four other nitrogen sources (ammonium sulphate, urea, peptone, and yeast extract) were also tested. Growth and cellulase production were greatly enhanced in fermentation using biomass of cyanobacterium as the nitrogen source. The use of cyanobacterial biomass as a nitrogen source also reduced the inhibitory effect of high concentrations of CaCl2 to growth of A. terreus and cellulase production. The addition of 0.3 g L-1 CaCl2 to the medium containing OPEFB fibre and cyanobacterial biomass further enhanced the cellulase production, though growth remained unchanged. The final FPase, CMCase, and β-glucosidase obtained in fermentation using 10 g L-1 OPEFB fibre and 6 g/L cynaobacterial biomass with the addition of 3 mM CaCl2 was 0.97 U mL-1, 14.1 U mL-1, and 10.4 U mL-1, respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: North Carolina State University
Keywords: Cyanobacterial biomass; Anacystis nidulans; Aspergillus terreus; Cellulase; Fermentation.
Depositing User: Najwani Amir Sariffudin
Date Deposited: 17 Jun 2014 01:33
Last Modified: 17 Jun 2014 01:33
URI: http://psasir.upm.edu.my/id/eprint/22233
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