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Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia


Sharif, Saeed (2010) Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia. Masters thesis, Universiti Putra Malaysia.


Feline coronavirus (FCoV) consisted of feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV) which cause a mild disease and fatal disease known as feline infectious peritonitis (FIP), respectively. There is neither effective vaccine, nor curative treatment for FIP, and a fast and reliable diagnosis would greatly assist in the management and control of the virus. In this study, a rapid and sensitive approach was established for detection of FCoV using reverse transcriptase polymerase chain reaction (RT-PCR) assay. In the attempt to improve the sensitivity and specificity of the assay, the RT-PCR assay was developed using SYBR Green 1 real-time RT-PCR. By using the RT-PCR assay the prevalence of FCoV was examined in 44 healthy and 28 sick cats suspected of FIP. Results showed FCoV infections in healthy and sick cats were of 84% and 89%, respectively. Phylogenetic analysis was performed on FCoV isolates obtained from 4 healthy and 10 sick cats. The sequence alignment revealed that the local isolates had 96% homology and fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia. In another study, a duplex RT-PCR assay was developed for detection of mRNS of FCoV in blood samples from 40 healthy and 10 FIP-suspected cats. The results showed that 67.5% of healthy cats were FCoV-positive and the viral mRNA was detected in 15% of animals. In testing of FIP-suspected cats, all cases were positive FCoV with detectable mRNA. Since it is assumed that the viral replication in blood is associated with FIP, the duplex RT-PCR assay is more specific for detection of FIP. However, detecting the viral mRNA by the duplex RT-PCR in asymptomatic cats should be interpreted in conjunction with other clinical findings.

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Additional Metadata

Item Type: Thesis (Masters)
Subject: Coronavirus infections - Malaysia
Call Number: FPV 2010 8
Chairman Supervisor: Siti Suri Arshad, PhD
Divisions: Faculty of Veterinary Medicine
Notes: Siti Suri Arshad, PhD
Depositing User: Haridan Mohd Jais
Date Deposited: 08 Jul 2013 02:42
Last Modified: 08 Jul 2013 02:42
URI: http://psasir.upm.edu.my/id/eprint/22079
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