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Characteristics of Progesterone Synthesis In Isolated Luteal Cells


Citation

Tan, Cheong Huat (1982) Characteristics of Progesterone Synthesis In Isolated Luteal Cells. Pertanika, 5 (2). pp. 224-228.

Abstract / Synopsis

Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis by these cells were examined. The isolated luteal cells were viable and functional, and respond to luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence of exogenous glucose, the rate of progesterone synthesis was 0.23 ng/ml/min. LH increased this rate 2-fold, and the duration of the initial linear phase of this reaction was sustained for up to 60 min, compared to the controls which were linear for only 15 min. This effect was dose-related: maximal stimulation was achieved with 1 J1g LH/ml, while a minimal but significant stimulation was elicited with 61 pg/ml. This steroidogenic activity was also increased by the addition of exogenous glucose and bovine serum albumin. These data serve to establish the optimum conditions for the use of single cells in studies pertaining to mechanism of LH action.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Environmental Studies
Keywords: Single luteal cells; progesterone synthesis; characteristics.
Depositing User: Nur Izyan Mohd Zaki
Date Deposited: 10 Nov 2009 14:41
Last Modified: 27 May 2013 14:58
URI: http://psasir.upm.edu.my/id/eprint/2115
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