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Enzyme and microbial technology: biotechnology of fats and oils.


Salleh, Abu Bakar and Abd. Razak, Che Nyonya and Wan Yunus, Wan Md. Zin and Ampon, Kamaruzaman and Basri, Mahiran (1991) Enzyme and microbial technology: biotechnology of fats and oils. In: Seminar Kebangsaan Menilai Pencapaian Penyelidikan IRPA Dalam Rancangan Malaysia ke-5 Sektor Perindustrian (20-24 Disember 1991) hosted in Universiti Utara Malaysia, Kedah, 20-24 Disember 1991, Universiti Utara Malaysia, Kedah. .


To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform synthetic reactions under controlled conditions. Our group have meticulously carried out a screening programme for microbes that can produce these enzymes. So far we have isolated over 50 microbial isolates exhibiting lipolytic activities on plate and managed to study in detail 3 fungi and 2 bacteria. To improve the versatility of these enzymes we are redesigning the enzymes with the hope of obtaining an analogue with better solubility in organic solvents. We have covalently attached aldehydes of various sizes and hydrophobicity onto the enzyme lipase, and the enzyme thus derivatised showed. enhanced catalytic activity and thermal stability compared with the native unmodified enzyme. Another approach is to immobilise the enzyme. The enzyme molecule is still, in principle, to another molecule. In most cases, the molecule attached yo the enzyme is usually insoluble, hence confering the derivatised enzyme a new property of insolubility. We have shown that enzymes can be immobilised by simple adsorption onto readily available resins.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Subject: Enzymes - Immobilized enzymes
Subject: Oils and fats - Biotechnology
Subject: Microbial biotechnology
Divisions: Faculty of Science and Environmental Studies
Keywords: Enzyme; Microbial technology; Biotechnology; Fats; Oils.
Depositing User: Samsida Samsudin
Date Deposited: 11 Jun 2013 06:21
Last Modified: 26 Aug 2014 08:50
URI: http://psasir.upm.edu.my/id/eprint/20856
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