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Enzyme assay for metal-reducing enzyme


Citation

Mohd. Yunus Abd. Shukor (2009) Enzyme assay for metal-reducing enzyme. UNSPECIFIED.

Abstract / Synopsis

An improved enzyme assay system for screening potential molybdenum-reducing microbes from the environment for the purpose of bioremediation is described. Said method includes contacting said crude enzyme preparation from the organisms with either nicotinamide adenine dinucleotide reduced from (NADH) or nicotinamide adenine dinucleotide phosphate in reduced from (NADPH), as electron donor substrates and laboratory-prepared phosphomolybdate as the electron acceptor substrate. The resultant blue intensity is mesured colorimetrically using a spectrophotometer. Said laboratory-prepared phosphomolybdate comprises of a mixture of molybdate and phosphate in the ratio of 10 moles molybdate to 1 to 5 moles phosphate.


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Additional Metadata

Item Type: Patent
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Keywords: Enzyme
Depositing User: Suzila Mohamad Kasim
Date Deposited: 21 May 2013 10:54
Last Modified: 21 May 2013 10:54
URI: http://psasir.upm.edu.my/id/eprint/20405
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