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Porcine specific real-time polymerase chain reaction (PCR) for halal verification


Khalid, Farihah Liyana and Mustafa, Shuhaimi and Che Man, Yaakob and Sazili, Awis Qurni and Azmi, Aida Azrina and Abdul Rahim, Raha (2009) Porcine specific real-time polymerase chain reaction (PCR) for halal verification. In: 3rd IMT-GT International Symposium on Halal science and Management 2009, 21-22 Dec. 2009, KLIA Sepang, Malaysia. (pp. 24-28).


A porcine-specific real-time polymerase chain reaction (PCR) detection of a region in the mitochondrial DNA was developed for Halal verification. A pair of porcine-specific primers was designed to generate a specific fragment of the porcine DNA. To verify the specificity of the primers designed, the primer pair was tested against ten samples to detect any possible crossreactions. The reaction only amplified porcine DNA at Ct 25.03 ± 0.33. The real-time PCR assay employed in this work was proven to be very sensitive with a low detection limit. The assay was done by preparing a 10-fold dilution series to determine the sensitivity of the reaction. The sensitivity threshold was up to 0.001ng porcine DNA.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Halal Products Research Institute
Publisher: Halal Products Research Institute, Universiti Putra Malaysia
Keywords: Real-time PCR; SYBR Green I; Porcine DNA; ND5 gene; Halal verification
Depositing User: Samsida Samsudin
Date Deposited: 29 Apr 2013 01:32
Last Modified: 03 Aug 2018 01:15
URI: http://psasir.upm.edu.my/id/eprint/19888
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