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Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones


Citation

Abdullah, Mohd Azmuddin and Ali, Abdul Manaf and Mahmood, Maziah and Lajis, Md Nordin and Ariff, Arbakariya (1998) Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones. Plant Cell, Tissue and Organ Culture, 54 (3). pp. 173-182. ISSN 0167-6857

Abstract

Morinda elliptica (Rubiaceae) cell suspension cultures were established in shake flask system for the production of anthraquinones. The optimized medium formulation for cell growth and anthraquinone production is proposed. Murashige and Skoog's basal medium (MS) was found to be the best medium, used in combination with 0.5 mg l-1 NAA and 0.5 mg l-1 kinetin. At the range of sucrose concentration tested (3-8% w/v), 8% was the best in enhancing both cell growth and anthraquinone production. A strategy to formulate growth and production medium by manipulating culture age and inoculum age, the type of medium formulation used to grow inoculum, incubation temperature and light intensity was established. By using 18 month old culture and 7 day old inoculum at incubation temperature of 27 ± 3°C, anthraquinone yield of 2.9 g l-1 and 4.5 g l-1, under illumination of 1200 lux and in the dark was obtained, respectively.


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Additional Metadata

Item Type: Article
Subject: Anthraquinones
Subject: Morinda - Biotechnology - Malaysia
Subject: Plant tissue culture
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1023/A:1006108717255
Publisher: Springer
Keywords: ; Auxin; Cytokinin; Inoculum age; Medium optimization; Morinda elliptica
Depositing User: Azwana Abdul Rahman
Date Deposited: 05 Nov 2011 11:34
Last Modified: 09 Dec 2015 09:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1023/A:1006108717255
URI: http://psasir.upm.edu.my/id/eprint/18379
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