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Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis


Abdul Manaf, Siti Ruhaya and Mustafa, Shuhaimi and Mat Amin, Nakisah and Ali, Abdul Manaf (2006) Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis. Journal of Applied Sciences, 6 (1). pp. 15-19. ISSN 1812-5654


Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330. © 2006 Asian Network for Scientific Information.

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Official URL or Download Paper: http://dx.doi.org/10.3923/jas.2006.15.19

Additional Metadata

Item Type: Article
Subject: Acanthamoeba - Malaysia
Subject: Genetic polymorphisms
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3923/jas.2006.15.19
Keywords: Acanthamoeba; DNA polymorphism; Genetic distance; RAPD-PCR
Depositing User: Azwana Abdul Rahman
Date Deposited: 25 Oct 2011 15:56
Last Modified: 09 Dec 2015 02:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/jas.2006.15.19
URI: http://psasir.upm.edu.my/id/eprint/18312
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